Our primary aim is assessment of specific interactions of biological systems with nanoparticles on different levels of biological organization.
Themes of our research
- Interaction of nanoparticles with lipid membranes: GUVs, SAXS, SISSI.
- Stress due to presence of nanoparticles: in vitro, in vivo, AOEB, lipid peroxidation, proteomics, feeding experiments.
- Unintentional internalization of nanoparticles: in vitro, AAS, X-ray beam lines.
- Biosensor approach: lipid vesicles, enzymes (AChE).
- In vitro approach: cell lines.
- Ex vivo approach: isolated hepatopancreas (Porcellio scaber).
- In vivo approach: model invertebrate organism (Porcellio scaber) – lab studies, environmental studies.
- Atomic absorption spectrometer (AAS) PerkinElmer Aanalyst 100.
- Equipment for sample preparation for AAS.
- Basic equipment for proteomics.
- Cell culture laboratory equipment (being established).
- Access to phase contrast microscope Nikon Eclipse T2000.
- Access to Zeiss AxioImager Z1 Microscope with ApoTome system and cameras (AxioCam MRm & AxioCam HRc).
- Access to SEM JEOL JSM 7500F.
- Access to histological laboratory with microtomes (Cryomicrotome Leica CM1850, Leica RM 2265, Microtome Reichert Autocut 2040).
- Access to equipment of CO NAMASTE and CO Nanocenter.
- Biological sample preparation for: conventional histology, SEM and FIB/SEM, Fluorescent microscopy, X-ray spectroscopy, IR spectroscopy.
- Proteomics of unicellular organisms and invertebrate tissue.
- Metal assimilation by invertebrates (terrestrial isopods).
- Single species toxicity testing by invertebrates (terrestrial isopods).
- Conducting experiments with artificial lipid membranes (vesicles) using light phase contrast microscopy, X-ray scattering techniques etc.
- Conducting experiments with enzymes using spectroscopy techniques.
- Image processing.
- Biomedical data analysis and machine learning.
- Programming (Matlab, Java, PHP, Excel VB).