An in vivo invertebrate bioassay of Pb, Zn and Cd stabilization in contaminated soil
The terrestrial isopod (Porcellio scaber) was used to assess the remediation efficiency of limestone and a mixture of gravel sludge and red mud as stabilizing agents of Pb, Zn and Cd in industrially polluted soil, which contains 800, 540 and 7 mg kg−1 of Pb, Zn and Cd, respectively. The aim of our study was to compare and evaluate the results of the biological and non-biological assessment of metal bioavailability after soil remediation. Results of a 14 d bioaccumulation test with P. scaber showed that that Pb and Zn stabilization were more successful with gravel sludge and red mud, while Cd was better stabilized and thus less bioavailable following limestone treatment. In vivo bioaccumulation tests confirmed the results of chemical bioaccessibility, however it was more sensitive. Biotesting with isopods is a relevant approach for fast screening of bioavailability of metals in soils which includes temporal and spatial components.