Barbara Drasler

[ email | bibliography ]

My main research activity is to study interactions of differently shaped nanoparticles with biological membranes using different methodological approaches.

My experiences in testing nanoparticles include following methods:

  • Light phase contrast microscopy and lipid vesicles of different types.
    In collaboration with Laboratory of Biophysics;

  • Small Angle X-ray Scattering technique (SAXS) and multilamellar vesicles (MLVs).
    In collaboration with with dr. Michael Rappolt;

  • in vivo and ex vivo studies with model digestive gland tissue of terrestrial invertebrate organism (Porcellio scaber, Isopoda, Crustacea);

  • Biochemical sensor system: enzyme tests with Acetylcholine Esterase.
    In collaboration with Departement of Biochemistry;

  • In vitro methods on different human cell lines: hepatocytes, astrocytes, lung epithelium cells; using methods High Content Screening Analysis (HCA) and Flow cytometry.
    In collaboration with CBNI, UCD, Dublin, Ireland;

  • Differential Scanning Calorimetry (DSC) and Flourescence Spectroscopy.
    In collaboration with Department of Biochemistry and Food chemistry;

  • Sample preparation for proteomics.

I graduated in Biology (September, 2010) from Biotechnical Faculty, University of Ljubljana, Slovenia.
The title of the thesis is: The effect of nanoparticles C60 and CoFe2O4 on artificial phospholipid membranes.

In October, 2010 I enrolled in the interdisciplinary doctoral study Bioscience, research area Nanosciences, coordinated by the Biotechnical Faculty, University of Ljubljana.

International activities:

Methods and machines I specialize in::

  • Light Microscopy and GUVs; cooperation in establishment of Standard Operation Procedure(SOP) for testing the biological potential of nanoparticles (biosensor-based, medium independent method);
  • Ex vivo membrane destabilisation studies (AO/EB staining) of digestive glands (Porcellio scaber);
  • Tissue culture laboratory establishemnt and introducing of different In vitro techniques.
2013
Effect of engineered TiO2 and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles
Simundic M, Drasler B, Sustar V, Zupanc J, Stukelj R, Makovec D, Erdogmus D, Hagerstrand H, Drobne D, and Kralj-Iglic V
[ pdf ] [ site ] BMC Veterinary Research

2012
Experimental evidence for the interaction of C-60 fullerene with lipid vesicle membranes
Zupanc J., Drobne D., Drasler B., Valant J., Iglic A., Kralj-Iglic V., Makovec D., Rappolt M., Sartori B, and Kogej K.
[ pdf ] [ site ] Carbon