My main research activity is to study interactions of differently shaped nanoparticles with biological membranes using different methodological approaches.
My experiences in testing nanoparticles include following methods:
- Light phase contrast microscopy and lipid vesicles of different types. In collaboration with Laboratory of Biophysics;
- Small Angle X-ray Scattering technique (SAXS) and multilamellar vesicles (MLVs). In collaboration with with dr. Michael Rappolt;
- in vivo and ex vivo studies with model digestive gland tissue of terrestrial invertebrate organism (Porcellio scaber, Isopoda, Crustacea);
- Biochemical sensor system: enzyme tests with Acetylcholine Esterase. In collaboration with Departement of Biochemistry;
- In vitro methods on different human cell lines: hepatocytes, astrocytes, lung epithelium cells; using methods High Content Screening Analysis (HCA) and Flow cytometry. In collaboration with CBNI, UCD, Dublin, Ireland;
- Differential Scanning Calorimetry (DSC) and Flourescence Spectroscopy. In collaboration with Department of Biochemistry and Food chemistry;
- Sample preparation for proteomics.
I graduated in Biology (September, 2010) from Biotechnical Faculty, University of Ljubljana, Slovenia. The title of the thesis is: The effect of nanoparticles C60 and CoFe2O4 on artificial phospholipid membranes.
In October, 2010 I enrolled in the interdisciplinary doctoral study Bioscience, research area Nanosciences, coordinated by the Biotechnical Faculty, University of Ljubljana.
- (February – May, 2011) Research at Centre for BioNano Interactions (CBNI), UCD , Dublin, Ireland: using In vitro methods HCA and Flow cytometry.
- (2010 – present) Research at SAXS, c/o ELETTRA (Sincrotrone Trieste), in colaboration with with dr. Michael Rappolt, Institute of Biophysics and Nanosystems Research (IBN), Austrian Academy of Sciences.
Methods and machines I specialize in::
- Light Microscopy and GUVs; cooperation in establishment of Standard Operation Procedure(SOP) for testing the biological potential of nanoparticles (biosensor-based, medium independent method);
- Ex vivo membrane destabilisation studies (AO/EB staining) of digestive glands (Porcellio scaber);
- Tissue culture laboratory establishemnt and introducing of different In vitro techniques.