Dr. Barbara Drašler

[ email | bibliography ]

I am currently a post doctoral researcher at the Bio-Nanomaterial group at the Adolphe Merkle Institute, University of Fribourg, Switzerland. (since January 2016)

My main research activity is to study interactions of differently shaped nanoparticles with artificial and biological membranes using different methodological approaches.

Individual scientific approaches and findings have been described in various papers on the assessment of nanomaterial-membrane interactions, and majority of the work has been included in my PhD thesis entitled Interactions of different nanomaterials with artificial and biological lipid membranes (Slovenian language). Here, the synthesis of the proposed mechanisms governing interactions between nanomaterials and membranes is discussed in more details and the effects, observed at different levels of biological organization, are compared.

My experiences in testing nanoparticles include following methods:

  • Light phase contrast microscopy and phospholipid vesicles (liposomes).
    In collaboration with Laboratory of Biophysics;

  • Small Angle X-ray Scattering technique (SAXS) and multilamellar vesicles (MLVs).
    In collaboration with with dr. Michael Rappolt;

  • In vivo and ex vivo studies with model digestive gland tissue of terrestrial invertebrate organism (Porcellio scaber, Isopoda, Crustacea);

  • Biochemical sensor system: enzyme tests with Acetylcholinesterase.
    In collaboration with Departement of Biochemistry;

  • Human red blood cells: isolation, sample preparation.
  • In vitro methods on different human cell lines: hepatocytes, astrocytes, lung epithelium cells; using methods High Content Screening Analysis (HCA) , Flow cytometry, Immunoblotting, Epifluorescence microscopy, Confocal Microscopy.
    In collaboration with CBNI, UCD, Dublin, Ireland;

  • Differential Scanning Calorimetry (DSC) and Flourescence Spectroscopy.
    In collaboration with Department of Biochemistry and Food chemistry;

  • Sample preparation for proteomics.

I graduated in Biology (September, 2010) from Biotechnical Faculty, University of Ljubljana, Slovenia.
The title of the thesis is: The effect of nanoparticles C60 and CoFe2O4 on artificial phospholipid membranes.

In October, 2010 I enrolled in the interdisciplinary doctoral study Bioscience, research area Nanosciences, coordinated by the Biotechnical Faculty, University of Ljubljana.

International activities:

  • (2014) Participation at the 7th International Nanotoxicology Congress (NanoTOX 2014) in Antalya. Student Fellowship Award.
  • (2013) 15-day Transnational Access (QualityNano Research Infrastructure) at Centre for Bionano Interactions (CBNI), UCD, Dublin, Ireland (Quality Nano fellowship).
  • (2012) Participation at the 6th Nanotoxicology Conference, September 4–7, 2012, Beijing China. Best Poster Award.
  • (2012) 10-day Transnational Access (QualityNano Research Infrastructure) at Centre for Bionano Interactions (CBNI), UCD, Dublin, Ireland (Quality Nano fellowship).
  • (February – May, 2011) Research at Centre for BioNano Interactions (CBNI), UCD , Dublin, Ireland: using In vitro methods HCA and Flow cytometry.
  • (2010 – present) Research at the Beamline SAXS, c/o ELETTRA (Sincrotrone Trieste S.C.p.A. di interesse nazionale, AREA Science Park, Basovizza, Trieste), in colaboration with with prof. dr. Michael Rappolt, Institute of Biophysics and Nanosystems Research (IBN), Austrian Academy of Sciences and at the Beamline SISSI (»Source for Imaging and Spectroscopic Studies in the Infrared«).

Methods and machines I specialize in::

  • Light Microscopy and GUVs; cooperation in establishment of Standard Operation Procedure(SOP) for testing the biological potential of nanoparticles (biosensor-based, medium independent method);
  • Ex vivo membrane destabilisation studies (AO/EB staining) of digestive glands (Porcellio scaber);
  • Tissue culture laboratory establishemnt and different in vitro techniques.
  • Human red blood cells: shape alterations upon contact with nanomaterials.
Comparative in vitro genotoxicity study of ZnO nanoparticles, ZnO macroparticles and ZnCl2 to MDCK kidney cells: Size matters
Kononenko V, Repar N, Marušič N, Drašler B, Romih T, Hočevar SB, Drobne D
[ site ] Toxicology in Vitro

Enhanced biocompatibility of TiO2 surfaces by highly reactive plasma
Junkar I, Kulkarni M, Drašler B, Rugelj N, Recek N, Drobne D, Kovač J, Humpolíček P, Iglič A, Mozetič M
[ site ] Journal of Physics D: Applied Physics

Influence of various sterilization procedures on TiO2 nanotubes used for biomedical devices
Junkar I, Kulkarni M, Drašler B, Rugelj N, Mazare A, Flašker A, Drobne D, Humpolíček P, Resnik M, Schmuki P, Mozetič M, Iglič A
[ site ] Bioelectrochemistry

Growth of a Novel Nanostructured ZnO Urchin: Control of Cytotoxicity and Dissolution of the ZnO Urchin
Imani R, Drašler B, Kononenko V, Romih T, Eleršič K, Jelenc J, Junkar I, Remškar M, Drobne D, Kralj-Iglič V, Iglič A
[ pdf ] [ site ] Nanoscale Research Letters

Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes
Santhosh PB, Drašler B, Drobne D, Erdani Kreft M, Kralj S, Makovec D, Poklar Ulrih N
[ pdf ] [ site ] International Journal of Nanomedicine

Fullerene up-take alters bilayer structure and elasticity: A small angle X-ray study
Drašler B, Drobne D, Sadeghpour A, Rappolt M
[ pdf ] [ site ] Chemistry and Physics of Lipids

Biological potential of nanomaterials strongly depends on the suspension media: experimental data on the effects of fullerene C60 on membranes
Drašler B, Drobne D, Poklar Ulrih N, Ota A
[ pdf ] [ site ] Protoplasma

Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles
Pajnič M, Drašler B, Šuštar V, Krek JL, Štukelj R, Šimundić M, Kononenko V, Makovec D, Hägerstrand H, Drobne D, Kralj-Iglič V
[ pdf ] [ site ] Journal of Nanobiotechnology

Bioavailability of cobalt and iron from citric-acid-adsorbed CoFe2O4 nanoparticles in the terrestrial isopod Porcellio scaber
Romih T, Drašler B, Jemec A, Drobne D, Novak S, Golobič M, Makovec D, Susič R, Kogej K
[ pdf ] [ site ] Science of the Total Environment

Chapter One – Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions
Keremidarska M, Hristova K, Hikov T, Radeva E, Mitev D, Tsvetanov I, Presker R, Drobne D, Drašler B, Novak S, Kononenko V, Eleršič K, Pramatarova L, Krasteva N
[ pdf ] [ site ] Advances in Planar Lipid Bilayers and Liposomes

Lipid Vesicle Shape Analysis from Populations Using Light Video Microscopy and Computer Vision
Zupanc J, Drašler D, Boljte S, Kralj-Iglič V, Iglič A, Erdogmus D, Drobne D
[ pdf ] [ site ] PLoS One

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes
Drašler B, Drobne D, Novak S, Valant J, Boljte S, Otrin L, Rappolt M, Sartori B, Iglič A, Kralj-Iglič V, Šuštar V, Makovec D, Gyergyek S, Hočevar M, Godec M, Zupanc J
[ pdf ] [ site ] International Journal of Nanomedicine

Effects of surface curvature and surface characteristics of carbon-based nanomaterials on the adsorption and activity of acetylcholinesterase
Mesarič T, Baweja L, Drašler B, Drobne D, Makovec D, Dušak P, Dhawan A, Sepčić K
[ pdf ] [ site ] Carbon

Effect of engineered TiO2 and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles
Šimundić M, Drašler B, Šuštar V, Zupanc J, Štukelj R, Makovec D, Erdogmus D, Hagerstrand H, Drobne D, and Kralj-Iglič V
[ pdf ] [ site ] BMC Veterinary Research

Experimental evidence for the interaction of C-60 fullerene with lipid vesicle membranes
Zupanc J, Drobne D, Drašler B, Valant J, Iglič A, Kralj-Iglič V, Makovec D, Rappolt M, Sartori B, Kogej K
[ pdf ] [ site ] Carbon