Dr. Veno Kononenko

[ email | bibliography ]

My main research activity experienced in studying biological effects of nanoparticles to mammalian cells, with a focus on studying cytotoxicity, genotoxicity, neurotoxicity and phospholipidosis (excessive intracellular accumulation of phospholipids). I study the effects of nanoparticles to different cellular prosecces (lipid metabolism, biogenesis of pulmonary surfactants…)

My experience with evaluating biological effects of nanoparticles include the following methods:

  • Cell culturing
  • Light and fluorescence microscopy
  • Spectrophotometry and spectrofluorometry
  • Flow cytometry
  • Cytotoxicity testing (MTT assay, Neutral red uptake assay, ATP luciferase assay, measurement of Reactive Oxygen Species – ROS, differential staining methods…)
  • Genotoxicity testing (Comet assay, Micronucleus assay)
  • Biochemical methods for the measurement of stress-related enzymes activity (catalase, glutathione S-transferase…)
  • Proteomic approaches (SDS-PAGE, 2D-PAGE, western blotting)
  • Biological characterization of nanoparticles

I graduated in Biotechnology (September, 2012) from Biotechnical Faculty, University of Ljubljana, Slovenia.

The title of the thesis is: The adaptation of comet assay with yeast Saccharomyces cerevisiae and isopod Porcellio scaber for testing genotoxicity of nanoparticles.
In October, 2012 I started the interdisciplinary doctoral study Bioscience, research area Nanosciences, coordinated by the Biotechnical Faculty, University of Ljubljana.

International activities:


Nicotinic acetylcholine receptor as a pharmacological target in lung cancer
Kononenko V, Bele T, Novak S, Križaj I, Drobne D, Turk T.
[ site ] Acta Biologica

Antibacterial and degradation properties of dialdehyded and aminohexamethylated nanocelluloses
Kokol V, Novak S, Kononenko V, Kos M, Vivod V, Gunde-Cimerman N, Drobne D
[ site ] Carbohydrate polymers

The interaction between the osteosarcoma cell and stainless steel surface, modified by high-fluence, nanosecond laser pulses
Hočevar M, Šetina B, Godec M, Kononenko V, Drobne D, Gregorčič P
[ site ] Surface & coatings technology

The first comprehensive safety study of Magnéli phase titanium suboxides reveals no acute environmental hazard
Jemec Kokalj A, Novak S, Talaber I, Kononenko V, Bizjak Mali L, Vodovnik M, Žegura B, Eleršek T, Kalčíková G, Žgajnar Gotvajn A, Kralj S, Makovec D, Čaloudova H, Drobne D
[ site ] Environmental science Nano

Phototoxicity of Mesoporous TiO2 + Gd Microbeads With Theranostic Potential
Kononenko V, Imani R, Repar N, Benčina M, Lorenzetti M, Erman A, Drobne D, Iglič A
[ site ] Advances in Biomembranes and Lipid Self-Assembly

Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells
Kononenko V, Erman A, Petan T, Križaj I, Kralj S, Makovec D, Drobne D
[ site ] Nanotoxicology

Multifunctional Gadolinium-Doped Mesoporous TiO2 Nanobeads: Photoluminescence, Enhanced Spin Relaxation, and Reactive Oxygen Species Photogeneration, Beneficial for Cancer Diagnosis and Treatment
Imani R, Dillert R, Bahnemann BW, Pazoki M, Apih T, Kononenko V, Repar N, Kralj-Iglič V, Boschloo G, Drobne D, Edvinsson T, Iglič A
[ site ] Small

Comparative in vitro genotoxicity study of ZnO nanoparticles, ZnO macroparticles and ZnCl2 to MDCK kidney cells: Size matters
Kononenko V, Repar N, Marušič N, Drašler B, Romih T, Hočevar SB, Drobne D
[ site ] Toxicology in Vitro

Bismuth film electrode for measuring Zn(II) under less acidic conditions in the presence of cell culture medium and ZnO nanoparticles
Romih T, Hočevar SB, Kononenko V, Drobne D
[ site ] Sensors and Actuators B: Chemical

Gold nanoparticles do not induce adverse effects on terrestrial isopods Porcellio scaber after 14-day exposure
Škarková P, Romih T, Kos M, Novak S, Kononenko V, Jemec A, Vávrová M, Drobne D
[ pdf ] [ site ] Acta Biologica Slovenica

Growth of a Novel Nanostructured ZnO Urchin: Control of Cytotoxicity and Dissolution of the ZnO Urchin
Imani R, Drašler B, Kononenko V, Romih T, Eleršič K, Jelenc J, Junkar I, Remškar M, Drobne D, Kralj-Iglič V, Iglič A
[ pdf ] [ site ] Nanoscale Research Letters

Nanoparticle interaction with the immune system
Kononenko V, Narat M, Drobne D
[ pdf ] [ site ] Archives of Industrial Hygiene and Toxicology

Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles
Pajnič M, Drašler B, Šuštar V, Krek JL, Štukelj R, Šimundić M, Kononenko V, Makovec D, Hägerstrand H, Drobne D, Kralj-Iglič V
[ pdf ] [ site ] Journal of Nanobiotechnology

Chapter One – Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions
Keremidarska M, Hristova K, Hikov T, Radeva E, Mitev D, Tsvetanov I, Presker R, Drobne D, Drašler B, Novak S, Kononenko V, Eleršič K, Pramatarova L, Krasteva N
[ pdf ] [ site ] Advances in Planar Lipid Bilayers and Liposomes