Dr. Veno Kononenko

[ email | bibliography ]

My main research activity is studying biological effects of nanoparticles to mammalian cells. My main interest is to study nanoparticle-cell interactions, with a focus on cytotoxicity, genotoxicity and the excessive intracellular accumulation of phospholipids (induction of phospholipidosis).

My experience with evaluating biological effects of nanoparticles include the following methods:

  • Cell culturing
  • Fluorescence microscopy
  • Spectrofluorometry
  • Flow cytommetry
  • Cytotoxicity testing (MTT assay, Neutral red uptake assay, measurement of reactive oxygen species, differential staining methods …)
  • Genotoxicity testing (Comet assay, Micronucleus assay)
  • Biochemical methods for the measurement of stress-related enzymes activity (catalase, glutathione S-transferase)
  • Proteomic approaches (SDS-PAGE, 2D-PAGE, western blotting)
  • Quantitative reverse transcription PCR (RT-qPCR)

I graduated in Biotechnology (September, 2012) from Biotechnical Faculty, University of Ljubljana, Slovenia.

The title of the thesis is: The adaptation of comet assay with yeast Saccharomyces cerevisiae and isopod Porcellio scaber for testing genotoxicity of nanoparticles.
In October, 2012 I started the interdisciplinary doctoral study Bioscience, research area Nanosciences, coordinated by the Biotechnical Faculty, University of Ljubljana.

International activities:

Awards:

2017
Phototoxicity of Mesoporous TiO2 + Gd Microbeads With Theranostic Potential
Kononenko V, Imani R, Repar N, Benčina M, Lorenzetti M, Erman A, Drobne D, Iglič A
[ pdf ] [ site ] Advances in Biomembranes and Lipid Self-Assembly

2017
Harmful at non-cytotoxic concentrations: SiO2-SPIONs affect surfactant metabolism and lamellar body biogenesis in A549 human alveolar epithelial cells
Kononenko V, Erman A, Petan T, Križaj I, Kralj S, Makovec D, Drobne D
[ pdf ] [ site ] Nanotoxicology

2017
Multifunctional Gadolinium-Doped Mesoporous TiO2 Nanobeads: Photoluminescence, Enhanced Spin Relaxation, and Reactive Oxygen Species Photogeneration, Beneficial for Cancer Diagnosis and Treatment
Imani R, Dillert R, Bahnemann BW, Pazoki M, Apih T, Kononenko V, Repar N, Kralj-Iglič V, Boschloo G, Drobne D, Edvinsson T, Iglič A
[ pdf ] [ site ] Small

2017
Comparative in vitro genotoxicity study of ZnO nanoparticles, ZnO macroparticles and ZnCl2 to MDCK kidney cells: Size matters
Kononenko V, Repar N, Marušič N, Drašler B, Romih T, Hočevar SB, Drobne D
[ pdf ] [ site ] Toxicology in Vitro

2016
Bismuth film electrode for measuring Zn(II) under less acidic conditions in the presence of cell culture medium and ZnO nanoparticles
Romih T, Hočevar SB, Kononenko V, Drobne D
[ pdf ] [ site ] Sensors and Actuators B: Chemical

2016
Gold nanoparticles do not induce adverse effects on terrestrial isopods Porcellio scaber after 14-day exposure
Škarková P, Romih T, Kos M, Novak S, Kononenko V, Jemec A, Vávrová M, Drobne D
[ pdf ] [ site ] Acta Biologica Slovenica

2015
Growth of a Novel Nanostructured ZnO Urchin: Control of Cytotoxicity and Dissolution of the ZnO Urchin
Imani R, Drašler B, Kononenko V, Romih T, Eleršič K, Jelenc J, Junkar I, Remškar M, Drobne D, Kralj-Iglič V, Iglič A
[ pdf ] [ site ] Nanoscale Research Letters

2015
Nanoparticle interaction with the immune system
Kononenko V, Narat M, Drobne D
[ pdf ] [ site ] Archives of Industrial Hygiene and Toxicology

2015
Effect of carbon black nanomaterial on biological membranes revealed by shape of human erythrocytes, platelets and phospholipid vesicles
Pajnič M, Drašler B, Šuštar V, Krek JL, Štukelj R, Šimundić M, Kononenko V, Makovec D, Hägerstrand H, Drobne D, Kralj-Iglič V
[ pdf ] [ site ] Journal of Nanobiotechnology

2015
Chapter One – Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions
Keremidarska M, Hristova K, Hikov T, Radeva E, Mitev D, Tsvetanov I, Presker R, Drobne D, Drašler B, Novak S, Kononenko V, Eleršič K, Pramatarova L, Krasteva N
[ pdf ] [ site ] Advances in Planar Lipid Bilayers and Liposomes